Rapid Identification and Classification of Psychrotrophic Lactic Acid Bacteria by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Rapid Identification of Anaerobic Bacteria Using Matrix Assisted Laser Desorption Ionisation Time-of-flight Mass Spectrometry

Identification and Classification of Rhizobia by Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry

Mass spectrometry (MS) has been widely used for specific, sensitive and rapid analysis of proteins and has shown a high potential for bacterial identification and characterization. Type strains of four species of rhizobia and Escherichia coli DH5α were employed as reference bacteria to optimize various parameters for identification and classification of species of rhizobia by matrix-assisted la...

Rapid Identification of Bacteria by Intact Cell Matrix-assisted Laser Desorption/ionisation Time of Flight Mass Spectrometry

Preliminary work has demonstrated that MALDITOF-MS spectra from intact cells of a range of different bacteria produced by different operators at three different laboratories are highly reproducible, provided appropriate protocols are followed. Since these spectra are representative of surface components present on the bacterial cell, they can be used to generate a unique pattern of peaks for ea...

Rapid Identification of the Foodborne Pathogen Trichinella spp. by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Human trichinellosis occurs through consumption of raw or inadequately processed meat or meat products containing larvae of the parasitic nematodes of the genus Trichinella. Currently, nine species and three genotypes are recognized, of which T. spiralis, T. britovi and T. pseudospiralis have the highest public health relevance. To date, the differentiation of the larvae to the species and geno...

Characterisation of bacteria by matrix-assisted laser desorption/ionisation and electrospray mass spectrometry.

Chemical analysis for the characterisation of micro-organisms is rapidly evolving, after the recent advent of new ionisation methods in mass spectrometry (MS): electrospray (ES) and matrix-assisted laser desorption/ionisation (MALDI). These methods allow quick characterisation of micro-organisms, either directly or after minimum sample preparation. This review provides a brief introduction to E...